Fig. 1
From: Structural and developmental dynamics of Matrix associated regions in Drosophila melanogaster genome
Effect of Stabilization and RNase A treatment on NuMat. A Resin less electron micrographs of unstabilized, stabilized and RNase A treated NuMat: The fine filaments seen in the stabilized NuMat are not seen in unstabilized NuMat. Instead, wide gaps with thicker fibers at the periphery are visible. The fine filaments seen in the stabilized NuMat collapse onto the peripheral lamina after RNase A treatment leaving wide gaps in the internal nuclear structure. (B) Confocal images of unstabilized, stabilized and RNase A treated NuMat: The nuclear panel shows presence of DNA as stained by DAPI. NuMat preparations are completely devoid of DNA. Internal lamin staining is visible in only in stabilized NuMat indicating it to be most intact