Fig. 1

Xenopus blastula explants can be reprogrammed to adopt any lineage. A Schematic of Waddington’s Landscape portraying lineage specification process. B Embryos stage 9,10,10.5,11,12, and 13 used to confirm developmental stages of blastula explants. C Schematic showing signaling molecules used to generate all 4 lineages. D-I RNA Seq TPM expression over time of (D) maternally provided pluripotency marker Pou5f3.3, (E) maternally provided Foxi2, (F) epidermal marker EpK, (G) neural marker Otx1, (H) mesoderm marker Brachyury(T) (I) endoderm marker Sox17. H Graphs are sums of S + L allele. Width of lines represents SEM of three biological replicates