Fig. 8

miR-133 could target and regulate ACVR1B, and miR-23b could target and regulate WNT10A. a The predicted sites of miR-133 binding to ACVR1B and miR-23b binding to WNT10A. b A luciferase reporter gene assay revealed that the overexpression of miR-133 significantly quenched wild-type ACVR1B fluorescence. c A luciferase reporter gene assay revealed that the overexpression of miR-23b significantly quenched wild-type WNT10A fluorescence. d RT–qPCR quantification indicated that the overexpression of miR-133 significantly reduced the mRNA expression level of ACVR1B. e RT–qPCR quantification indicated that the overexpression of miR-23b significantly reduced the mRNA expression level of WNT10A. f Western blot analysis indicated that the overexpression of miR-133 significantly reduced the protein expression level of ACVR1B. g Western blot analysis indicated that the overexpression of miR-23b significantly reduced the protein expression level of WNT10A. h Analysis of the relative protein expression of ACVR1B. i Analysis of the relative protein expression of WNT10A. **p < 0.01, *p < 0.05, ns p > 0.05