Fig. 6

Subcellular localisation of U2B″-GFP. (A) Prediction results of subcellular localisation of U2B″ protein in different species. The larger the circle, the more likely it is to be positioned in that place. (B) Rice protoplast were selected as observation objects. U2B″-GFP and mCherry (nuclear localisation marker) were co-transferred into protoplast to observe the localisation of proteins. The white arrow marks the nucleoli. The experiment was repeated more than three times. (C) Nuclear localisation prediction of rice U2B″ protein. (D) Prediction of transmembrane structure of rice U2B″ protein