Fig. 4

Multiple sequence alignment and phylogenetic analysis of the LsPIN1 protein of L. secalinus and PIN1 proteins from other species, and subcellular localization of LsPIN1 protein in tobacco leaf cells. a The full-length amino acid sequences of 30 PINs were used to build the phylogenetic tree with MEGA 7.0 software. The LsPIN1 was marked with the red circle. b Multiple sequence alignment of LsPIN1, OsPIN1, and AtPIN1 was conducted using ClustalX 1.81 with default parameters. c Transcript levels of LsPIN1 in horizontal rhizome tips and upward rhizome tips. Error bars were the standard errors (SE). Different lowercase letters represented significant differences (P < 0.05). d Transient expression of 35S:LsPIN1-GFP and 35S:GFP in tobacco leaf cells. After 48 h of transformation, a confocal microscope was used to observe the green fluorescence signal. Bars = 20 μm