Fig. 6
From: Identification of LsPIN1 gene and its potential functions in rhizome turning of Leymus secalinus
Gravitropic and phototropic responses in LsPIN1-overexpressing seedlings. a Phenotypes of LsPIN1-overexpressing seedlings and Col-0 seedlings were characterized at 2, 3, and 5 d following gravistimulation by rotating their culture dishes 135° from the vertical. b The turning angle of the root tips in (a). c Phenotypes of Col-0 and LsPIN1 transgenic seedlings (L1, L2, and L3) after 7 d under simulated microgravity conditions on a monoaxial clinostat. d The gravitropic angle was measured for 10 roots per line using ImageJ software. e The phenotypes of transgenic and Col-0 seedlings were characterized after being placed in a dark environment with 12 h of unilateral light stimulation. The arrow indicated the orientation of light. Scale bars: 1 cm. f The bending angle of hypocotyl was measured by ImageJ software. Average bending angle measurements were taken from 20 seedlings. All experiments were repeated three to four times, and the results of each replication were similar. Error bars indicated SE calculated from the results of three independent experiments. Different lowercase letters represented significant differences (P < 0.05)