Fig. 4

Functional conservation of root active CLE peptides in tomato and Arabidopsis. A. Effect of Arabidopsis and tomato orthologous CLE peptides on the primary root length (PRL). B. Representative confocal images of tomato primary root tips grown on mock or indicated SlCLE peptide containing medium. The cell walls are stained with calcofluor white. Red and green arrows indicate root width columella length, respectively C-F. Quantification of the indicated root tip morphology characteristics from images show in B. Letters indicate different statistical groups (ANOVA, followed by post-hoc Tukey test). G. Primary root length of wild type and Slclv2 mutant grown in presence of SlCLE6/19p and SlCLE24p. H. Representative confocal images of wild type and Slclv2 primary root tips grown on mock or indicated SlCLE peptide containing medium. The scale bars in B and H correspond to 100 μm