Fig. 2

Phelotrupes auratus transcript analysis strategy. We performed genome sequencing and transcriptome analysis, followed by gene prediction. blastx was performed between P. auratus transcripts and human proteins in public databases to annotate the genes that were identified through RNA-Seq as having differential expression between Nara Park and Cape Toi samples. Then, we converted the transcript IDs from the P. auratus RNA-Seq to human NCBI IDs and constructed an assignment table. Finally, we performed gene enrichment analysis and molecular network analysis using the public database and determined molecular interactions in polyembryogenesis. The illustrations of experimental tools, humans and machines (https://togotv.dbcls.jp/ja/pics.html) are licensed under Creative Commons Attribution 4.0 International License (CC BY 4.0) (http://creativecommons.org/licenses/by/4.0/deed.en)