Fig. 3

Imprinted genes upregulated in neuroendocrine cells in the Ximerakis, Lipnick [29] whole mouse brain dataset. A GSEA for imprinted genes upregulated in the neuroendocrine cells. In the analysis, genes are sorted by strength by which they mark this neuronal cluster (sorted by Log2FC values) indicated by the bar (middle). Fold change values are displayed along the bottom of the graph. The genes are arrayed left (strongest marker) to right and blue lines mark where imprinted genes fall on this array. The vertical axis indicates an accumulating weight, progressing from left to right and increasing or decreasing depending on whether the next gene is an imprinted gene or not. The p-value represents the probability of observing the maximum value of the score (red dashed line) if the imprinted genes are distributed randomly along the horizontal axis. The q-value for this analysis was significant at 0.0036. B Dot plot of imprinted genes upregulated in the ‘Neuroendocrine cells’ plotted across all identified cell types (Abbr. in Table 3). Imprinted genes were plotted in chromosomal order. Size of points represented absolute mean expression; colour represented the size of the Log2FC value for the cell identity group (e.g., neuroendocrine cells) vs. all other cells. Unique colour scales are used for MEGs (red/orange) and PEGs (blue). Where a gene was not expressed in a cell type, this appears as a blank space in the plot