Fig. 1

Phylogenetic analysis of HAK families across Medicago, Arabidopsis, Glycine max and Oryza sativa. Full-length protein sequences of HAKs were constructed using MEGA-X based on the Neighbor-Joining (NJ) method; bootstrap was 1,000 replicates. Four clusters (I, II, III, IV) are subdivided into two sub-clusters A and B. The green solid pentagrams, orange solid pentagrams, hollow circles, red triangle and black square represent HAK proteins from M. truncatula (Mt), M. sativa (Ms), A. thaliana (At), G. max (Gm) and O. sativa (Os); Phylogenetic trees were designed using MEGA7.0 according to the maximum likelihood method and performed bootstrap testing with 1000 replicates