Fig. 8

Quantification of gene expression levels of selected HAK genes from M. truncatula using RT-qPCR. Expression level of MtHAK genes under NaCl and mannitol stresses. Actin gene was used as house-keeping gene in RT-qPCR. Expression level of each gene at 0 h for each treatment was set as value of 1 and their expression levels at each time point were relative to 0 h. Data are average of three independent biological samples ± SE and vertical bars indicate standard deviation. ** indicates P < 0.01, and * indicates P < 0.05