Fig. 9

Quantification of gene expression levels of selected HAK genes from M. sativa using RT-qPCR. A: Expression level of MsHAK genes under NaCl and mannitol stress. Actin gene was used as house-keeping gene in RT-qPCR. Expression level of each gene at 0 h for each treatment was set as value of 1 and their expression levels at each time point were relative to 0 h. Data are average of three independent biological samples ± SE and vertical bars indicate standard deviation. ** indicates P < 0.01, and * indicates P < 0.05. B. Correlation analysis of RT-qPCR and transcriptome (NaCl and mannitol) data for MsHAK genes. Pearson’s value indicates the Pearson correlation coefficient