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Fig. 6 | BMC Genomics

Fig. 6

From: Benchmarking full-length transcript single cell mRNA sequencing protocols

Fig. 6

Comparative analysis of cell similarity, transcript type, length and unique genes between scRNA-seq data generated with NEB®, Takara®, G&T and SS3 protocols. A Spearman correlation coefficient (SCC) between all cells from the same protocol. B Proportion of different transcript types in each library. Non-coding transcript types constitute e.g. tRNA, snRNA, snoRNA, miRNA, miscRNA, lincRNA. C Bar-chart illustrating total unique genes captured across all cells by each protocol. D Venn diagram visualizing overlap of total captured genes between protocols, and genes captured uniquely in each protocol. E Average gene transcripts according to length (bp) across all tested protocols. Significance level cut-off: ns:p < 0.05, *:p <  = 0.05, **:p <  = 0.01, ***:p <  = 0.001, ****:p <  = 0.0001

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