Fig. 4

miR-24-3p is a candidate regulator of gene expression in human CRC. A Heatmap showing the log2 fold change for miRNAs differentially expressed (> 1000 RPMMM in either condition, fold change > 1.5x, p-adj < 0.05) between TCGA primary colon adenocarcinoma (n = 371) and non-tumor tissue (n = 8). Color intensity represents the log2 fold change. B Plot of the -log10 (p-value) of target site enrichment, calculated by miRhub (cons2) for each differentially expressed miRNA from (A), using the list of genes that are differentially expressed (DESeq2 expression > 1000 normalized counts in either condition, fold change > 1.5x, p-adj < 0.05) in the opposite direction of the miRNA. MiRNAs within the same family were grouped together under the same name. MiRNAs with target site enrichment p-value < 0.05 shown in red. (C) Expression (log2 RPMMM) of the 17 miRNAs from (B) in matched TCGA primary colon adenocarcinoma (n = 8) and non-tumor (n = 8) tissue (two-tailed Welch t-test). Lines connect tissue samples collected from the same patient. D Venn diagram for miRNAs of interest identified by the mouse enteroid and TCGA analyses (Created with BioRender.com). MiRNAs in red are upregulated. MiRNAs in blue are downregulated. Paralogs are listed as one miRNA. E Log2 fold change of miR-24-3p expression (RPMMM) across TCGA tumor types (n = 23). Colon (COAD) and rectal (READ) adenocarcinomas in red. Circle size represents the geometric mean (RPMMM) of miR-24-3p for each tumor type. Tumor types highlighted by blue boxes have Benjamini–Hochberg padj < 0.05. *p < 0.05, **p < 0.01, ***p < 0.001