Fig. 2

Immunofluorescence (IF) assay and miRNA analysis of rabbit BATs at four growth stages. A IF of BATs at four stages. Two different magnifications are presented in the IF assay (200 μm and 20 μm). The nucleus were stained using DAPI. The blue and green signals represent the nucleus and UCP1 proteins, respectively. B Construction of circRNA- and lncRNA-related ceRNA networks during BAT whitening in rabbits. C Differential analysis of miRNAs. The scatter plot showed miRNAs with log2FC > 1 and < -1 in the six comparisons. The red and orange points show the miRNAs with a p-value < 0.05. D Validation of nine differentially expressed miRNAs (DEmiRNAs) using RT-qPCR. The red and black lines represent the miRNA-seq and RT-qPCR, respectively. The expression was normalized to the U6 gene and D0. The data shows the means of three independent experiments. Two technical replicates were set for one individual experimental replicate