Fig. 4

Genome-wide identification and differential analysis of circular RNAs (circRNAs) of BATs in rabbits. A Genomic distribution of circRNAs in a pooled RNA sample using circRNA-seq. B Venn diagram analysis of the circRNAs identified from circRNA-seq and whole-transcriptome data. C The Venn diagram analysis of expressed circRNAs among different growth stages. D Divergent primers amplify circRNAs. The IDs of circRNA were listed in Table S6. “M” represents “DNA marker”. The bands with expected length size were extracted and subjected to Sanger sequencing. The gels were cropped from Figure S5. E Sanger sequencing confirms head-to-tail splicing. The splicing mode and the GT/AG splicing signal of one circRNA were shown. The black arrows are pointing the back-splicing sites of the other eight circRNAs. F K-means clustering of DECs based on expression levels.