Fig. 7

Validation of ocu-miR-378-5p-related ceRNA network. A Ocu-miR-378-5p-related ceRNA network. The green, purple, red, and blue nodes represent circRNA, lncRNA, miRNA, and mRNAs. B Dual-luciferase reporter assay validates the interaction between ocu-miR-378-5p and SLC15A3. The data in the dual-luciferase reporter assay shows the means of three independent experiments. The error bars show the standard errors of means. C Dual-luciferase reporter assay validates the interaction between ocu-miR-378-5p and MSTRG.16862.1. The data in the dual-luciferase reporter assay shows the means of three independent experiments. The error bars show the standard errors of means. D Validation of full length of novel_circ_0013792 by Sanger sequencing. “M” represents “DNA marker”. The bands with expected length size were extracted and subjected to Sanger sequencing. The gels were cropped according to the red lines in Figure S9. The black arrows point the BSJ sites of novel_circ_0013792 in Sanger sequencing. E Assembly of the full length of novel_circ_0013792. The blue and italicized bases show the sequence amplified by DIV1 primers and DIV2 primers, respectively. The black arrows show the binding sites and amplification directions of DIV1 primers. The red arrows show the binding sites and amplification directions of DIV2 primers (F) Dual-luciferase reporter assay validates the interaction between ocu-miR-378-5p and novel_circ_0013792. The data in the luciferase reporter assay shows the means of six independent experiments. The error bars show the standard errors of means