Table 2 Nucleotide sequence, location, and parameters of the primers designed in this study. ON861824 refers to the complete mitochondrial genome of Bactrocera dorsalis 2
Primer | Sequence 5’-3’ | Location in ON861824 | Tm (°C) | GC% |
|---|---|---|---|---|
trnI_trnQ_F | TGAATTGCCTGACAAAAGGG | 3–22 | 53.5 | 45.0 |
trnI_trnQ_R | GGTATGAACCCAGTAGCTTA | 215–234 | 51.1 | 45.0 |
rrnS_trnI_F | GCTGGCACAAATTTAACCAA | 14,787–14,806 | 52.0 | 40.0 |
rrnS_trnI_R | CCCTTTTGTCAGGCAATTCA | 3–22 | 53.5 | 45.0 |
Mito_F | TGACAAAAGGGTTACCTTGATAGGG | 12–36 | 56.6 | 44.0 |
Mito_R | ACCCAGTAGCTTAATTAGCTTATCT | 203–227 | 53.4 | 36.0 |