Fig. 5

METTL3-mediated m6A regulates alternative splicing of genes functioning in muscle development. A A schematic of cell culturing and treatment for sequencing. B qPCR results showed that METTL3 expression was significantly reduced in si-METTL3 cells. C Genome browser tracks showing RNA-seq read coverage at METTL3 gene body in si-METTL3 cells and control cells. D The count of different type of DAS events. E Venn diagram of SE-DAS events. F The heatmap of PSI level in SE-DAS events. Each row represents one AS events. G The exon skipping events were validated by RT-PCR (left panel). PCR primers anneal to exons flanking the skipped exon. Gel electrophoresis images were captured by Tanon-3500 digital gel image system. The inclusion level of SE events was quantified using ImageJ software (right panel), and calculated as: upper band intensity / (upper band intensity + lower band intensity)