Fig. 2

The differential expression analysis of genes. A Mean number of expressed genes of three replicates identified at each development stage. B The number of differentially expressed genes (DEGs) for comparison of each stage with the next stage. C Cluster analysis of gene expression. Embryos at different stages were clustered into two distinct clusters. One cluster contains replicates of Em1d and Em2d, two-stages. While Em3d, Em4d, and Em5d into the second cluster. This indicates a major shift in the gene expression from 3 stage onward