Genomic diversity and comprehensive taxonomical classification of 61 Bacillus subtilis group member infecting bacteriophages, and the identification of ortholog taxonomic signature genes

Table 2 Characteristics of BSP members of the newly proposed New subfamily1 and New subfamily2

Family/Class		Siphoviridae		Caudoviricetes
Proposed subfamily		New subfamily1		New subfamily2
Member Genus		New genus1	New genus2	Spbetavirus	New genus7	New genus8	New genus9
Member Species		1. Ray17	2. 000TH010	1. SPbeta	2. BUCT082	3. Goe11	6. phi3T
			3. SPP1			4. Goe12
			4.049ML001/049ML003			5. Goe13
Inter-genera similarity %		21.3–22.1		41.1–64.7
Phylogenomy		Non-monophyletic		Monophyletic
Proteome-based cluster/phylogeny		Distinct cluster/Monophyletic		Distinct cluster/Monophyletic
Subfamily-unique genes/proteins using 30% identity and 50% coverage cutoff		Major capsid, HTH DNA binding domain and portal protein, and recombinase, transcription factor, terminase large subunit, and transcriptional repressor		Integrase, tyrosine recombinase, lysogeny pheromone peptide, arbitrium peptide, UV damage repair protein, ssDNA specific exonuclease, SOS response-associated peptidase, Lysis-lysogeny pheromone receptor
Phylogenetic analysis	MCP	Monophyletic		MCP is absent in all members
	DNA polymerase	DNA polymerase is absent in all members		Non-monophyletic
	Tail fiber protein	Not monophyletic		Monophyletic except phi3T which lacks the protein
	PGA hydrolase	Monophyletic except SPP1 that lacks the protein		Monophyletic except phi3T which lacks the protein
	Peptidoglycan binding protein	The protein is absent in all members		Monophyletic
	Genome-size group	Medium (39.33–55.46 kb)		Large (124.29–163.32 kb)

ISSN: 1471-2164