Fig. 3
From: A role for worm cutl-24 in background- and parent-of-origin-dependent ER stress resistance
Making hemizygote mutants for RH-seq. RH-seq requires hemizygote hybrids (purple) from crosses between mutants of one background (red) and wild-types of another (blue). Top: arrays (large ovals) harboring the Mos1 transposon (green) and heat-shock-inducible transposase enzyme gene (orange) in the red background come together into one strain. Center: after heat shock, a transposon copy integrates into the genome (straight black line) of an egg of the red background, which is fertilized by a wild-type male of the blue background. Bottom: the resulting F1 hybrids are hemizygous throughout the soma and are used as input into a sequencing-based tunicamycin resistance assay