Fig. 2

Schematic of REDfly-EnhancerAtlas comparisons performed in this study. See text and Methods for details. A REDfly enhancers under 2000 bp in length were selected and tested for overlap against each of the 21 EnhancerAtlas Drosophila enhancer sets. Similar comparisons were made subsequently after filtering out all enhancers defined by STARR-seq assays. B REDfly enhancers were placed into tissue-specific sets after discarding all sequences longer than 1000 bp (600 bp for some sets). Eleven such sets were tested for overlap with eleven tissue-matched sets from EnhancerAtlas. Each EnhancerAtlas set was then split into multiple individual sets with the data from just a single component experiment (“subsets”), and each subset was tested for overlap against the corresponding REDfly tissue-matched set. C EnhancerAtlas data are based on genome-wide assays, while REDfly enhancers are drawn from individual reporter gene experiments. Therefore, we expect the majority of REDfly enhancers for a given tissue to comprise a subset of the EnhancerAtlas enhancers for the matched tissue