Fig. 4

Cumulative and Isoform specific expression of gene families associated with hemicellulose synthesis. Gene families: Uridine Diphosphate Glucose Dehydrogenase (UGD) in Figure a, Uridine Diphosphate Glucuronic Acid Epimerase (UGE) in Figure b, Uridine Diphosphate Xylose Epimerase (UXE) in Figure c, Uridine Diphosphate Glucose 4,6-dehydratase (RHM) in Figure d, Uridine Diphosphate Xylose Synthase (UXS) in Figure e, Uridine Diphosphate Glucose 4-Epimerase (GALE) in Figure f, and Uridine Diphosphate Glucose Apiose/Xylose Synthase (UAXS) in Figure g. Abbreviations, TI: Top Internode; MI: Middle Internode; BI: Bottom Internode; 1st Visible Dewlap Leaf: L1; 5th Visible Dewlap Leaf: L5; R: Root. The bar chart displays the cumulative expression of all genes within a gene family. Letters above each bar indicate the presence of a significant difference between values within the same genotype. Error bars +/− 1 S.D. from biological triplicates. The heat map plots the Log₂ TPM values for each individual gene family member. Green displays higher expression values, yellow for mid-range values and red for lower range expression values. Letters within the heatmap indicate the presence of a significant difference between values within an individual gene, within the same genotype. Significance was calculated via one way ANOVA, with the post-hoc Tukey’s T-test to separate statistically dissimilar groups. Statistical analysis was measured separately within each genotype