Fig. 9
From: Characterization of the REVEILLE family in Rosaceae and role of PbLHY in flowering time regulation
Overexpression of PbLHY delayed flowering in Arabidopsis. (a) Flowering phenotypes of representative control plants and PbLHY-OE lines grown under long-day conditions for six weeks. (b) and (c) The number of days and rosette leaves were recorded in control and PbLHY-OE lines when the first flower was visible. *** p < 0.001 vs. vector control by t test. (d) Expression of PbLHY in control and PbLHY-OE lines. (e-g) Expression of AtGI, AtCO, and AtFT in control and PbLHY-OE lines. Transcript levels were measured by qRT–PCR assay in 12-day-old seedlings under long-day conditions and normalized to AtACT. The highest expression level of each gene was set to 1 for standardized calculation. Error bars represent the standard error. White and black areas indicate light and darkness, respectively