Table 4 The primers for PCR genotyping
Primer name | Primer sequences (5'-3') | Size (bp) |
|---|---|---|
IL3-F | GGGACTCCAAGCTTCAATCA | 544 |
IL3-R | TGGAGGAGGAAGAAAAGCAA | Â |
Sry-F | TGGGACTGGTGACAATTGTC | 402 |
Sry-R | GAGTACAGGTGTGCAGCTCT | Â |
Wnt1-Cre-F | CAGCGCCGCAACTATAAGAG | 350 |
Wnt1-Cre-R | CATCGACCGGTAATGCAG | Â |
P1 | GGCACATTTCAGACACCAGAG | WT: 499 |
P2 | CTGTAGGTGTGAATTCGAGGC | Mid1-cKO: 557 |
P3 | CAGTTAGTCAAGGAAAAGCATCAC | WT: 344 |
P4 | GAGCATTTAAATTGTATCCTGTCA | Mid1-cKO: 466 |
P5 | GGCACATTTCAGACACCAGAG | WT: 1721 |
P6 | TGCCAAAATACGGACGAACCAT | Mid1-cKO: 511 |