Fig. 5

Sequence features of P. falciparum lncRNAs. a 26 RNA families (22 unique) were identified that aligned to annotated lncRNA sequences: 13 snoRNAs, 5 RUFs, 4 tRNAs, ribonuclease P, 2 signal recognition peptides and U6 spliceosomal snRNA using Rfam [39]. b Visual representation and IDs of lncRNAs containing snoRNAs, snRNAs and tRNAs. c LncRNA lengths ranged from 200 to 7452 bp and the distribution of lengths differed between subtypes (AS:antisense to). d LncRNAs were AT-rich with an average GC content of 15.97% and the distribution of GC content differed between subtypes (AS:antisense to). e 16 lncRNAs were identified as putatively coding by sequence feature-dependent coding potential analysis with CPC2 [40]. The distributions of the measures used to determine coding potential (peptide length, isoelectric point and Fickett score) are presented for putative coding and noncoding lncRNA annotations