Fig. 5

Histograms showing the size distribution of the different types of structural variants (SV) identified by analysis of long read (LR) or short read (SR) genomic sequence data for 5 inbred strains. The lines show the continuous density distribution for each type of SV as determined by Gaussian kernel estimation. Deletions are the most abundant type of SV, and the top graph indicates that many more deletions are identified using LR genomic sequence, especially when the deletion size is either < 1kB or > 10kB. The density lines in the middle graph show that LR sequence analysis also identifies more inversions, especially those with a size > 1kB. In the bottom graph, it appears that more duplications were detected with SR sequence. However, similar to what was observed with deletions (see Fig. 4B-D), the increased number of duplications may result from improper alignment of SR genomic segments, which occurs because of the limitations of SR genomic sequencing technology. Of importance, the small number of very large SVs must be experimentally verified