Table 2 Experiment tested
From: An SPRI beads-based DNA purification strategy for flexibility and cost-effectiveness
Test component | Buffer composition | Work condition | |||
|---|---|---|---|---|---|
Vol.beads/Vol.DNA | Separating time (min) | Incubated temperature | Incubated time (min) | ||
PEG 8000 | 16.3% PEG 8000, 10 mg/ml beads, 2 M NaCl, 16.3 mM MgCl2 | 1.2× | 5 | RT | 10 |
18% PEG 8000, 10 mg/ml beads, 2 M NaCl, 16.3 mM MgCl2 | 1.2× | 5 | RT | 10 | |
20% PEG 8000, 10 mg/ml beads, 2 M NaCl, 16.3 mM MgCl2 | 1.2× | 5 | RT | 10 | |
21.4% PEG 8000, 10 mg/ml beads, 2 M NaCl, 16.3 mM MgCl2 | 1.2× | 5 | RT | 10 | |
22.6% PEG 8000, 10 mg/ml beads, 2 M NaCl, 16.3 mM MgCl2 | 1.2× | 5 | RT | 10 | |
Beads (SPION particles) | 10 mg/ml beads, 20% PEG 8000, 2 M NaCl, 16.3 mM MgCl2 | 1.2× | 5 | RT | 10 |
5 mg/ml beads, 20% PEG 8000, 2 M NaCl, 16.3 mM MgCl2 | 1.2× | 5 | RT | 10 | |
2.5 mg/ml beads, 20% PEG 8000, 2 M NaCl, 16.3 mM MgCl2 | 1.2× | 5 | RT | 10 | |
1.25 mg/ml beads, 20% PEG 8000, 2 M NaCl, 16.3 mM MgCl2 | 1.2× | 5 | RT | 10 | |
0.675 mg/ml beads, 20% PEG 8000, 2 M NaCl, 16.3 mM MgCl2 | 1.2× | 5 | RT | 10 | |
Separating time | 1.25 mg/ml beads, 20% PEG 8000, 2 M NaCl, 16.3 mM MgCl2 | 1.2× | 5 | RT | 10 |
1.25 mg/ml beads, 20% PEG 8000, 2 M NaCl, 16.3 mM MgCl2 | 1.2× | 2 | RT | 10 | |
1.25 mg/ml beads, 20% PEG 8000, 2 M NaCl, 16.3 mM MgCl2 | 1.2× | 1 | RT | 10 | |
Incubated temperature | 1.25 mg/ml beads, 10% PEG 8000, 1.25 M NaCl, 10 mM MgCl2 | 1.2× | 5 | 28 °C | 10 |
1.25 mg/ml beads, 10% PEG 8000, 1.25 M NaCl, 10 mM MgCl2 | 1.2× | 5 | 25 °C | 10 | |
1.25 mg/ml beads, 10% PEG 8000, 1.25 M NaCl, 10 mM MgCl2 | 1.2× | 5 | 18 °C | 10 | |