Fig. 1

SM/J islets contain four subpopulations of β-cells. (A) Data analysis pipeline identifies subpopulations of β-cells and integrates bulk and single cell RNA sequencing data. (B) Single cell RNA sequencing UMAP plot of islet tissue. VSMC – vascular smooth muscle cell, VEC – vascular endothelial cell. (C) Single cell RNA sequencing UMAP of 4 subpopulations of β-cells. (D) Bootstrap analysis quantifies relative proportions of β-cell subpopulations across cohorts. Bar plot represents actual proportion, error bars illustrate 95% confidence interval. Cell type composition estimates with non-overlapping confidence intervals are significantly different. (E) Ternary plot illustrates subtype composition of Beta 1–3 in high-fat SM/J mice. HF20–20wk high-fat female, HF30–30wk high-fat female, HM20–20wk high-fat male, HM30–30wk high-fat male, LF20–20wk low-fat female, LM20–20wk low-fat male