Fig. 4

Pdyn is differentially expressed between and within β-cell subtypes. (A)Pdyn is differentially expressed in high-fat males in bulk RNAseq data. (B) Differential expression of β-cell-specific genes across all β-cells between 20wk and 30wk high-fat males in single cell RNAseq data. (C) Percent of β-cells in 20wk and 30wk high-fat males expressing β-cell-specific genes. (D) Total expression of Pdyn across all β-cells in 20- and 30wk high-fat males. (E) Expression of β-cell-specific genes between Beta 1 and Beta 2 cells across all individuals. (F) Percent of Beta 1 and Beta 2 cells expressing β-cell-specific genes across all individuals. (G) Total Pdyn expression in Beta 1 and Beta 2 cells across all individuals. (H) Expression of β-cell-specific genes in Beta 1 cells between 20wk and 30wk high-fat males. (I) Total Pdyn expression in Beta 1 cells across in 20wk and 30wk high-fat males. **p-value < 0.01 in FDR corrected pairwise comparison. HF20–20wk high-fat female, HF30–30wk high-fat female, HM20–20wk high-fat male, HM30–30wk high-fat male, LF20–20wk low-fat female, LM20–20wk low-fat male, LF30–30wk low-fat female, LM30–30wk low-fat male. Blue genes are significantly under-expressed in comparison, red genes are significantly over-expressed. Vertical golden lines indicate threshold for significance based on average log fold change, horizontal line indicates threshold for significance based on Bonferroni-corrected p-value