Fig. 2

(A) - Per base sequence quality (read 1) of libraries prepared using Standard illumina V3-V4 primers with 20% PhiX spike-in. (B) - Per base sequence quality (read 2) of libraries prepared using Standard illumina V3-V4 primers with 20% PhiX spike-in. (C) – Per base sequence quality (read 1) of libraries prepared using ‘N’ (0–10) spacer-linked V3-V4 primers without PhiX Spike-in. (D) - Per base sequence quality (read 2) of libraries prepared using ‘N’(0–10) spacer-linked V3-V4 primers without PhiX Spike-in