Fig. 4
From: Genome-wide identification of Kanamycin B binding RNA in Escherichia coli
Kanamycin B binding measurements to RNA using MST. a Binding curve generated by MST for Kanamycin B binding to the positive and negative control RNAs. Error bars were calculated from three independent biological repeats with duplicate measurements of each sample. A signal-to-noise ratio of more than 5 is desirable, while more than 12 reflects an excellent assay. b Binding curve generated by MST for binding of Kanamycin B to tRNA, 5sRNA or ncRNA. c Binding affinity of 28 enriched RNAs with Kanamycin B measured by MST