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Fig. 3 | BMC Genomics

Fig. 3

From: Potential key genes involved in metabolic resistance to malathion in the southern house mosquito, Culex quinquefasciatus, and functional validation of CYP325BC1 and CYP9M12 as candidate genes using RNA interference

Fig. 3

Expression of target genes (mean ± SEM (n ≥ 3)) three days after interference via dsRNA injection detected by real-time quantitative PCR. DsP450s and dsRFP were injected into 3-day-old female adults of Cx. quinquefasciatus from the Sebring strain. (A) Relative expression level of target genes, CYP325BC1 and CYP9M12, in dsP450-injected female adults was significantly suppressed compared to dsRFP-injected female adults. (B) Relative expression level of target genes, CYP325BC1 and CYP9M12, in dsP450-injected female adults was significantly suppressed compared to uninjected female adults from the Sebring strain. A Student’s t-test was used for significance analysis. *P < 0.05; **P < 0.01; ***P < 0.001

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