Fig. 3From: Potential key genes involved in metabolic resistance to malathion in the southern house mosquito, Culex quinquefasciatus, and functional validation of CYP325BC1 and CYP9M12 as candidate genes using RNA interferenceExpression of target genes (mean ± SEM (n ≥ 3)) three days after interference via dsRNA injection detected by real-time quantitative PCR. DsP450s and dsRFP were injected into 3-day-old female adults of Cx. quinquefasciatus from the Sebring strain. (A) Relative expression level of target genes, CYP325BC1 and CYP9M12, in dsP450-injected female adults was significantly suppressed compared to dsRFP-injected female adults. (B) Relative expression level of target genes, CYP325BC1 and CYP9M12, in dsP450-injected female adults was significantly suppressed compared to uninjected female adults from the Sebring strain. A Student’s t-test was used for significance analysis. *P < 0.05; **P < 0.01; ***P < 0.001Back to article page