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Fig. 4 | BMC Genomics

Fig. 4

From: Single-cell transcriptome analysis and in vitro differentiation of testicular cells reveal novel insights into male sterility of the interspecific hybrid cattle-yak

Fig. 4

Detection of the proliferation of spermatogenic cells cultured in vitro. A The average EDU fluorescence intensity of yak and cattle-yak spermatogenic cells, respectively. B RT-qPCR detection of the germ cell mark genes, GFRA1, THY1, UCHL1, KI67, c-kit, STR8 and SYCP3 expression in cattle-yak and yak spermatogenic cells cultured in vitro, respectively. C Western Blotting detection of the germ cell mark proteins, PLZF, GFRA1, c-kit and DDX4 expression in cattle-yak and yak spermatogenic cells cultured in vitro, respectively. The expression of β-actin was served as control

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BMC Genomics

ISSN: 1471-2164

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