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Fig. 7 | BMC Genomics

Fig. 7

From: Genome-wide identification of Aux/IAA and ARF gene families reveal their potential roles in flower opening of Dendrobium officinale

Fig. 7

Analysis of the interaction between Aux/IAA and ARF proteins using a yeast two-hybrid assay. DoARF2, 17 and 23 were cloned into the pGADT7 vector; DoIAA1, 6, 10 and 13 were cloned into the pGBKT7 vector. All recombinants were transformed into yeast strain AH109 and plated on the following double or quadruple selection media: SD/-Leu/-Trp, SD/-Leu/-Trp/-His/-Ade and SD/-Leu/-Trp/-His/-Ade + X-α-gal. An empty vector was used as the control

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