Fig. 1

scRNA-seq analysis of human dental pulp and reference tissues from healthy adults. (a) UMAP visualization of all integrated tissues, cell clusters are colored by tissue of origin. (b) UMAP visualization of unbiased cluster classification identifying 19 cell clusters with cell type annotation. (c) Feature plots of fibroblast markers identifying the cell population within clusters 0, 2, 3, 7 and 18. (d) Bar plot of the fraction of cells that compose each cluster shown in c, colored by tissue of origin. (e) Principal component analysis plot of fibroblasts performed using average gene expression for each sample. (f) Heatmap of the top 500 differentially expressed genes that contributed to PC1 and PC2 in e, set 1 shows upregulated genes in DP fibroblasts. (g) Dot plot of curated genes of interest retrieved from heatmap set 1, the complete list of genes is provided in Supplementary Table 1