Fig. 7

RNO_CIRCpedia_4214 facilitated macrophage polarization to the M2 type. A Sanger sequencing confirmed the back-splice junction sites of RNO_CIRCpedia_4214. B RNA FISH showed that RNO_CIRCpedia_4214 was predominantly localized in the cytoplasm. RNO_CIRCpedia_4214 probes were labeled with Cy-3. The nuclei were stained with DAPI. Scale bar, 10 µm. C–E The expression levels of RNO_CIRCpedia_4214, rno-miR-667-5p, Msr1, iNOS and Arg1 mRNA or protein were detected by RT-qPCR and western blotting, respectively, in macrophages transfected with siRNA, as well as controls. F The putative binding sequences between RNO_CIRCpedia_4214 and RNO-miR-667-5p. G Dual-luciferase reporter assays were performed to verify whether RNO-miR-667-5p was a target of RNO_CIRCpedia_4214. The data are presented as the mean ± SD (n = 3). *P < 0.05; **P < 0.01; ***P < 0.001