Fig. 1

(A) Schematic of an annotated skink mitogenome (Sphenomorphus incognitus, MH329292) displaying primer binding sites of long-range PCR Fragments 1 and 2 (green outer lines). Note that the fragments narrowly overlap. (B) Overview of lab workflow: (1) first-round PCR with tailed primers targeting primer binding sites and resulting in universal adapters attached to PCR fragments; (2) second-round PCR targeting the universal adapters and resulting in indexed fragments; (3) equimolar pooling of asymmetrically indexed fragments (note that Fragment 1 and 2 of the same sample received an identical index pair); (4) separate library preparation protocols for PacBio and Oxford Nanopore sequencing