Table 1 Primer sequences (5’ to 3’) developed for this study. Universal adapter sequences denoted by lower case letters. Primers were HPLC purified and ordered with a 5’ block (NH4–C6) to ensure first-round products were not ligated to SMRTbell adapters. Position refers to the 3’ end of the primer on the Sphenomorphus incognitus mitochondrial genome (Fig. 1) with base 1 referring to tRNA-Phe and continuing through 12 and 16 S rRNA.

From: Highly-multiplexed and efficient long-amplicon PacBio and Nanopore sequencing of hundreds of full mitochondrial genomes