Highly-multiplexed and efficient long-amplicon PacBio and Nanopore sequencing of hundreds of full mitochondrial genomes

Table 2 Summary of coverage per amplicon or per sample produced by NGSpeciesID. Note that reduced recovery of ONT samples due to non-optimized BUPs. We also calculated the percent recovery after removing samples that were in the F subpool as these were only included optimistically despite having no clear post-PCR gel band (see text for details)

Group	Samples/ Fragments Input	Samples/ Fragments Recovered	Recovery After Accounting for F pool	Mean Coverage	SD	Min	Max
ONT Fragment 1	335	142 (42.3%)		51.5x	30.4x	1x	197x
ONT Fragment 2	461	224 (48.5%)		79.8x	61.9x	1x	305x
ONT Fragment 1 and 2	549	116 (NA)¹
ONT Fragment 1 or 2	549	250 (45.5%)
PacBio Fragment 1	571	438 (76.7%)	83.7%	13,80.3x	1,500.1x	1x	15,261x
PacBio Fragment 2	588	487 (82.8%)	89.0%	1,420.2x	4,147.1x	1x	54,970x
PacBio Fragment 1 and 2	677	266 (39.2%)	44.5%
PacBio Fragment 1 or 2	677	649 (95.9%)	97.9%

¹Not comparable because the ONT pool did not contain equal quantities of Fragments 1 and 2 for each sample

ISSN: 1471-2164