Fig. 1

Identification of injury-responsive enhancer elements in neonatal mouse heart during regeneration. A Heatmaps of H3K27ac ChIP-seq signals (normalized by corresponding input samples) at genomic regions around the center of IREs (from -5 kb to + 5 kb) in the P1 + 1.5 dpi and P1 + 1.5dps samples. Each row represents a single enhancer (N = 1,852). Red stands for high density, and blue for low signal. B Boxplots showing expression level changes of IRE closest genes in P1 mouse hearts upon injury, and other genes are used as background. P-value: Wilcoxon rank-sum test. C, D Metagene plots (C) or heatmaps (D) of H3K27ac ChIP-seq signals (normalized by corresponding input samples) in the genomic regions around the TSSs (from -5 kb to + 5 kb) of IREs nearest genes in P1 + 1.5dps and P1 + 1.5dpi mouse hearts. E Heatmaps of H3K27ac ChIP-seq signals (normalized by corresponding input samples) around the centers of IREs (identified in P1 mouse hearts) (from -5 kb to + 5 kb) in the P8 + 1.5dpi and P8 + 1.5dps groups. Each row represents a single enhancer (N = 1,852). Red stands for high density, and blue for low signal. F Boxplots showing expression level changes of IRE (identified in P1 mouse hearts) closest genes in P8 mouse hearts upon injury compared to the other genes. P-value: Wilcoxon rank-sum test. G, H Metagene plots (G) or heatmaps (H) of H3K27ac ChIP-seq signals (normalized by corresponding input samples) around the TSSs (from -5 kb to + 5 kb) of IRE (identified in P1 mouse hearts) nearest genes in P8 + 1.5dps and P8 + 1.5dpi mouse hearts. I Genome browser view showing the increase in H3K27ac ChIP-seq signal at an IRE (with brown shadow) and expression level of nearby gene (Ctss) in P1 and P8 mouse hearts upon injury. Red stands for 1.5 dps, and blue is for 1.5 dpi. Arrow under the gene stands for transcription direction. J Boxplot showing difference of IRE induction level quantified by log2 fold change of H3K27ac ChIP-seq signals (1.5 dpi versus 1.5 dps) between P1 and P8 mouse hearts. P-value: Wilcoxon signed-rank test. K Boxplot displaying difference of gene activation level measured by log2 fold change of expression level (1.5 dpi versus 1.5 dps) between P1 and P8 mouse hearts. P-value: Wilcoxon signed-rank test