Fig. 2

AP-1 and ETS motifs are enriched in cardiac IREs in both zebrafish and mice. A Heatmaps of H3K27ac ChIP-seq signals (normalized read counts) at genomic regions around the center of zebrafish IREs (from -5 kb to + 5 kb) in the regenerating and uninjured groups. Each row represents a single enhancer (N = 646). Red stands for high density, and white for low signal. B Boxplots showing expression level changes of IRE closest genes and other genes (as background) in zebrafish hearts upon injury. P-value: Wilcoxon rank-sum test. C Metagene plots (left) or heatmaps (right) showing H3K27ac ChIP-seq signals (normalized read counts) at the genomic regions around the TSSs (from -5 kb to + 5 kb) of IRE nearest genes in uninjured and regenerating zebrafish hearts. D Scatter plots of motif enrichment analyses for zebrafish (left) and mouse (right) IREs during heart regeneration. AP-1 motifs are highlighted in blue, and ETS motifs are in red. Each dot stands for a single transcription factor binding motif. Red dotted line represents a p-value cutoff of 1 × 10^–10. E, F Line plots showing expression levels of genes encoding AP-1 (E) or ETS (F) transcription factors upon cardiac injury in zebrafish and mice. P-values: paired Student’s t-test. Mouse ETS genes up-regulated notably and their orthologs in zebrafish are highlighted in (F). G Donut plots showing the counts and percentages of different types of IREs relative to AP-1 or ETS motif (orange: only with AP-1 motif, blue: only with ETS motif, red: with both AP-1 and ETS motif, grey: with neither AP-1 nor ETS motif) in zebrafish (left) and mouse (right) hearts. H, I Boxplots of H3K27ac ChIP-seq signals (log2 transformed) for zebrafish and mouse IREs with or without AP-1 (H) or ETS (I) motif in uninjured (left) and regenerating (middle) samples, as well as log2 fold changes (regenerating versus uninjured) of H3K27ac signals (right). P-values: Wilcoxon rank-sum test. The median values are shown at the top of graphs