Fig. 5

The esBAF complex oppositely regulates occupancy of fragile nucleosomes and overlapping dinucleosomes. a-b. Mononucleosome occupancy visualized over H3K4me1 (a, GSE31039 [37, 58]) and H3K36me3 ChIP-seq peaks (b, GSE31039 [37, 58]). n = 3 merged replicates per condition. Samples were not gel-extracted but were bioinformatically selected for fragments between 135–165 bp. c-d. Overlapping dinucleosome occupancy following Smarca4, Smarca5, or EGFP control knockdown as in a-b. n = 3 merged replicates per condition. Samples were gel-extracted between 200–300 bp and bioinformatically selected for fragments between 230–270 bp. e. Proposed model integrating regulation of OLDNs and fragile nucleosomes by esBAF.