Fig. 4

Chromatin Environment of Functional Regulatory Elements. A Tag density plots of mean intensity and heatmaps of ChIP-Seq or ATAC-Seq performed in human CD4+ T cells plotted against STARR-Seq–identified Enhancers, NREs, and ‘All’ open peaks from input [35]. Nucleosome location in resting CD4+ T cells imputed from NucleoATAC [43]. Tag density of mean signal intensity displayed for Histone enrichment, and of imputed nucleosome position. Reads are centered at CRE peak center ‘C’. B Proportion of functional CD4+ STARR-Seq Enhancers and NREs within ChromHMM Segmentation of 25 imputed groups [4]. Other cell groups displayed as percent of total. C RELI enrichment logP-value of available enhancer predictions in FANTOM5 database [44]. Coverage ratio is the proportion of target sites overlapping all sites. D RELI enrichment logP-value of STARR-Seq identified elements across available DNase-Seq experiments, E H4K1me1 experiments, and F H3K27ac experiments [44]