Fig. 7

Gene expression of poised genes enriched in beta cells with a non-beta cell lineage. A Evaluating alpha repressed genes (Fig. 6A) across alpha, alpha transdifferentiated, and beta cell transcriptomes. The great majority (83.6%) of genes repressed in alpha cells showed intermediate expression in alpha transdifferentiated cells, and highest expression in beta cells, further validating that alpha cells are poised to become beta cells, with a subset (16.4%) of those genes required for the transition. B Evaluating delta repressed genes (Fig. 6C) across delta, delta transdifferentiated, and beta cell transcriptomes. Around half (50.18%) of genes repressed in delta cells showed intermediate expression in delta transdifferentiated cells, and highest expression in beta cells, validating that delta cells – to a lesser extent than alpha – are also poised to become beta cells, with the remainder of genes (49.82%) required for the transition