Fig. 9

Putative enhancer detection overlap between the three cell types. A First-pass overlap of unfiltered putative enhancers called with our novel package, epiRomics. Open chromatin regions in at least one cell type were crossed against two informative histone marks—H3k27ac and H3k4me1 – and transcription factor binding data to call putative enhancer regions. A total of 28,647 regions were identified (Dataset-S3). 39.8% of putative enhancer calls had chromatin accessible to all three cell types, suggestive of pancreatic endocrine cell development and maintenance involvement. The overlap of enhancer calls with open chromatin between any two cells type was 8.51%—18.9%. Between 1.89%—9.94% of calls were unique to one cell type alone. B First-pass enhancer calls were filtered against the curated FANTOM5 database delineating all identified enhancers in the mouse genome. This resulted in a much more conservative list of 3,535 regions identified (Dataset-S4). The distribution of enhancers unique or common between cell types remained comparable, with 43.2% identified across all three cell types, and 1.53%—10.1% unique to a cell type. C Confirming an enhancer on the second intron of Slc30a8, identified in a previous study, with 14 sites of co-binding from multiple transcription factors. D Confirming an a promoter-proximal enhancer (~ 1 kb upstream) of the gene that codes for the transcription actor Pdx1, with 9 sites of co-binding from multiple transcription factors