Fig. 3

RT-PCR validation of a subset of HEITs. Left panels: schematic representations of each analysed transcript (depicted in blue) with respect to its corresponding canonical form, as defined in the Ensembl database (depicted in orange). Forward and Reverse oligonucleotide primers used in RT-PCR experiments to validate the distinguishing features of the novel transcripts are depicted as red arrows in the higher magnification insets (circles). Right panels: Agarose gel electrophoresis of RT-PCR products. A Validation of a PLA2G5 transcript containing a novel exon. B Validation of a GRK1 transcript containing a novel exon. C Validation of a PRPH2 transcript containing a novel exon skipping event. The RT-PCR product corresponding to the canonical PRPH2 transcript is indicated in orange, whereas the product corresponding to the novel isoform is in blue. D Validation of a KIAA1549 transcript that harbours a novel exon. E Validation of a MAK transcript that contains an alternative last coding exon). F Validation of an RDH5 transcript that is connected with the adjacent BLOC1S1 transcriptional unit. G Validation of a MERTK transcript that contains a novel exon. Please note that the images showing the RT-PCR results of GRK1 (B) and KIAA1549 (D) were cropped and reorganized for the sake of clarity (source data are shown in Supplementary Fig. S2). L, 1000 bp ladder; B, Blank; BL, Blood; R, Retina; R1, Retina1; R2, Retina2; PO, Podocytes; F, Fibroblasts