Table 2 List of novel ORFs predicted from novel HEITs that significantly alter the corresponding annotated ORF

ATF6

MSTRG.2704.13

NM_007348.4

Connections with other transcriptional units

179 amino acids added at the N-terminus

BBS1

MSTRG.7009.36

NM_024649.5

Connections with other transcriptional units

127 amino acids added at the N-terminus

CERKL

MSTRG.23909.5

NM_201548.5

Alternative 3' coding exon

31 amino acids added at the C-terminus

KCNV2

MSTRG.40364.4

NM_133497.4

Alternative 3' coding exon

92 amino acids added at the C-terminus

LRAT

MSTRG.31449.4

NM_004744.5

Alternative 5' coding exon

20 amino acids added at the N-terminus

PRCD

MSTRG.18347.19

NM_001077620.3

Alternative 5' coding exon

Generation of a different protein product

RBP3

MSTRG.4643.1

NM_002900.3

Alternative 5' coding exon

45 amino acids added at the N-terminus

RBP4

MSTRG.5275.2

NM_006744.4

Alternative 3' coding exon

67 amino acids added at the C-terminus

CNGA3

MSTRG.22934.5

NM_001298.3

Skips canonical exon 3

38 amino acids removed

CWC27

MSTRG.32403.4

NM_005869.4

Novel exon predicted in intron 11

7 amino acids added

KIAA1549

MSTRG.38096.12

NM_001164665.2

Novel exon predicted in intron 12

16 amino acids added

MAK

MSTRG.34222.19

NM_001242957.3

Alternative 3' coding exon

2,163 amino acids added at the C-terminus

PDE6C

MSTRG.5276.12

NM_006204.4

Alternative 5' coding exon

14 amino acids added at the 5' end

PRDM13

MSTRG.35426.2

NM_021620.4

Skips canonical exon 2

44 amino acids removed

RGR

MSTRG.5132.34

NM_001012720.2

Skips canonical exons 2 and 3

52 and 45 amino acids removed

UNC119

MSTRG.17051.7

NM_005148.4

Skips canonical exon 2

38 amino acids removed

ZNF408

MSTRG.6569.5

NM_024741.3

Alternative 5' coding exon

9 amino acids added at the N-terminus

ZNF423

MSTRG.15493.3

NM_001379286.1

Alternative 5' coding exon

80 amino acids added at N-terminus

ZNF423

MSTRG.15493.5

NM_001379286.1

Novel exon predicted in intron 1

23 amino acids added