Fig. 6

(a) Acrosome reacted sperm arising from the CTC fluorescence assay. AR pattern, with dull fluorescence over the whole head except for the thin, bright punctate band of fluorescence in the equatorial segment (arrow). (b) Uncapacitated sperm arising from the chlortetracycline fluorescence assay. F pattern, with fluorescence over the whole head (arrow). (c) Capacitated sperm arising from the chlortetracycline fluorescence assay. B pattern, with a fluorescence-free band in the post acrosomal region (arrow). (d) The effect of PRDX6 on fertility capacity of frozen-thawed sperm. (e) Mitochondrial activity of sperm by JC-1 staining. The data were analyzed by t test *P < 0.05, **P < 0.01, *** P < 0.001